Journal: Chemistry & Biodiversity

Article Title: Integrative Assessment of Teucrium Extracts: Phytochemical Profile, Nutritional‐Mineral Composition, Biological Activities, and Toxicological Assessment In Vitro and In Vivo

doi: 10.1002/cbdv.71839

Figure Lengend Snippet: Cytotoxic activity of Teucrium extracts against human cancer and normal cell lines.

Article Snippet: Human cell lines: HeLa, MDA‐MB‐231, A549, A375, LS 174T, and MRC‐5, were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Activity Assay

Journal: bioRxiv

Article Title: VASH1 phosphorylation regulates tubulin detyrosination via CNP/PRKG1α signaling in DRG neurons

doi: 10.64898/2026.10.14.759141

Figure Lengend Snippet: Comparison of the activity of sfGFP-VASH1-SVBP (WT) and the mutant (S337E) enzyme complexes (50 pM) on taxol-stabilized HeLa tyrosinated (Tyr) microtubules measured by immunofluorescence in BRB40 buffer supplemented with 50 mM KCl as in . ( A ) Representative images of tyrosinated (Tyr, red) and detyrosinated (dTyr, turquoise) α-tubulin pools of microtubules after 30 min incubation in the absence (control) or presence of the indicated enzyme complexes. Scale bar, 10 μm. ( B ) Quantification of tyrosinated (Tyr) and detyrosinated (dTyr) tubulin signals. Each point represents a microtubule, at least 270 microtubules across 3 experiments done with 3 independent sets of recombinant enzymes were analyzed. Data are represented as mean ± SD. Statistical significance was determined using Kruskal-Wallis test, ****P < 0.0001.

Article Snippet: HeLa S3 cells (CLS Cell Lines Service GmbH) were first cultivated ∼5 days in adherent cultures, and then used to inoculate 2 liters of suspension culture that were cultured during ∼14 days at 65 rpm, 37°C, 5% CO 2 in a New Brunswick S41i incubator (Eppendorf).

Techniques: Comparison, Activity Assay, Mutagenesis, Immunofluorescence, Incubation, Control, Recombinant